ELISA method is a widely utilized immunoassay for quantitating and detecting proteins, hormones, peptides, cytokines, antibodies, and other drugs along with their metabolites. ELISA methods are effective in quantitatively detecting any molecule, or antigen, that can be ascertained by an antibody. For example, ELISA methods are used in pregnancy testing, infectious disease identification, and detection of cytokines, and soluble receptor proteins, etc. Due to the precision, sensitivity, assay speed, and ease of quantitation.
ELISA methods are highly sensitive and specific, allowing you to quantify proteins, hormones, drugs and other compounds that can be detected by an antibody. ELISA method development is a common choice for diagnostic and research applications due to the precision, sensitivity, assay speed, and ease of quantitation.
Sandwich elisa analysis methods are the most commonly used format for ELISA assay types. These assay formats have several variants, but the most commonly employed format uses an anti-analyte/capture antibody conjugate bound to the capture surface. The capture surface is also coated with a mix of anti-analyte labeled antibodies (substrate antibodies) and which avidly bind their corresponding capture proteins. After incubation and washing of the test sample with this substrate antibody-analyte complex, any remaining unbound sample components are washed off and allowed to react with reagents that have been pre-conjugated with a chromophore such as HRP or alkaline phosphatase.
Fas ligand, also known as fas fasl or CD95L, is a homotrimeric human type II transmembrane protein. The Fas ligand gene (FASLG) on chromosome 1q24.3 encodes the Fas ligand protein. Cytotoxic T lymphocytes express FasL on their surfaces that belong to the TNF family of proteins. By binding to its receptors, FasL induces apoptosis in cells. A matrix metalloproteinase MMP-7 cleaves membrane-bound FasL and generates a soluble FasL.
The Fas ligand or FasL (CD178 or CD95L) is a homotrimeric human type II transmembrane protein. Cytotoxic T lymphocytes express FasL on their surfaces that belong to the TNF family of proteins. By binding to its receptors, FasL induces apoptosis in cells. A matrix metalloproteinase MMP-7 cleaves membrane-bound FasL and generates a soluble FasL. This dataset is associated with cancer progression and immune system regulation.
Fas ligand or FasL (CD178 or CD95L) is a homotrimeric human type II transmembrane protein. The Fas ligand gene (FASLG) on chromosome 1q24.3 encodes the Fas ligand protein. Cytotoxic T lymphocytes express FasL on their surfaces that belong to the TNF family of proteins. The Fas ligand gene (FASLG) on chromosome 1q24.3 encodes the Fas ligand protein. Cytotoxic T lymphocytes express FasL on their surfaces that belong to the TNF family of proteins. By binding to its receptors, FasL induces apoptosis in cells.
Fas ligand or FasL (CD178 or CD95L) is a homotrimeric human type II transmembrane protein. The Fas ligand gene (FASLG) on chromosome 1q24.3 encodes the Fas ligand protein. Cytotoxic T lymphocytes express FasL on their surfaces, which belong to the TNF family of proteins. By binding to its receptors, FasL induces apoptosis in cells. A matrix metalloproteinase MMP-7 cleaves membrane-bound FasL and generates a soluble form of this protein.